Impact of iron-manganese modified Camellia oleifera shell-based biochar on the anaerobic digestion performance and microbial community structure of sludge

doi:10.3969/j.issn.2097-0706.2024.08.006

Abstract

Abstract:

Hydrolysis process limits the anaerobic digestion （AD） rate of sludge. Supplementing exogenous biochar （BC） can effectively boost methane production by overcoming the limitation in hydrolysis. The iron-manganese modified biochar （Fe-Mn-BC） derived from the residual shells of woody oil crops， specifically Camellia oleifera shells， is studied. SEM， FTIR， XPS and XRD are employed to characterize the material， and its impacts on sludge AD performance， methane yield and microbial community structure are explored. The study results demonstrate that since Fe-Mn-BC possesses a porous structure， iron and manganese particles can load onto the BC surface in various forms of oxides. The addition of Fe-Mn-BC elevates methane production. When the total solid mass fraction of Fe-Mn-BC reaches 80 mg/g， cumulative gas production peaks at 301.59 mL/g， marking a 45.27% increase compared to that of the control group. Microbial community analysis reveals that Fe-Mn-BC enriches the abundance of archaeal communities， including Crenarchaeota， Candidatus_Methanomethylicus and Candidatus_Methanofastidiosum. These communities play crucial roles in promoting the hydrolysis of organic matters and enhancing the methane production， indicating that Fe-Mn-BC not only enriches functional microbial communities such as methanogenic bacteria， but also effectively improves the efficiency of sludge AD. Furthermore， this method presents a resource utilization solution for Camellia oleifera shells.

Key words: anaerobic digestion, sludge, Camellia oleifera shell, iron-manganese modified biochar, microbial community, methane, hydrolysis

CLC Number:

TK09：X705

LUO Kun, ZHU Yi, HUANG Jing, LI Hui. Impact of iron-manganese modified Camellia oleifera shell-based biochar on the anaerobic digestion performance and microbial community structure of sludge[J]. Integrated Intelligent Energy, 2024, 46(8): 41-49.

Figures/Tables 10

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参数	单位	接种污泥	基质污泥
pH值		7.34±0.06	7.42±0.03
TS	g/L	13.62±0.13	79.85±0.46
VS	g/L	5.36±0.04	42.58±0.32
SCOD	mg/L	472.83±26.29	6 183.74±89.15
可溶性蛋白质量浓度	mg/L	43.57±2.49	395.76±2.81
可溶性多糖质量浓度	mg/L	34.59±6.83	402.65±8.22

样品	组成元素的质量分数/%					S_BET/（m²·g^-1）
样品	C	N	O	Fe	Mn	S_BET/（m²·g^-1）
BC	86.43	4.74	8.84	—	—	2.81
Fe-Mn-BC	65.03	—	21.25	7.50	1.92	18.29

试验组	Shannon指数		Simpson指数		Chao1指数		覆盖度
试验组	细菌	古菌	细菌	古菌	细菌	古菌	细菌	古菌
H0	2.558	1.164	0.112	0.361	53.5	8.0	0.999	1.000
H1	2.495	1.234	0.114	0.330	54.5	7.0	0.999	1.000
H2	2.651	1.254	0.100	0.330	51.0	8.0	0.999	1.000
H3	2.681	1.323	0.100	0.313	53.0	8.0	0.999	0.999
H4	2.746	1.462	0.098	0.272	57.0	10.0	0.999	0.999
H5	2.708	1.433	0.099	0.298	56.3	9.0	0.999	0.999